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Expression vector

From Academic Kids

An expression vector is a relatively small DNA molecule that is used to introduce and express a specific gene into a target cell. Once the expression vector is inside the cell, the protein that is encoded by the gene is produced by the cellular transcription and translation machinery.

Expression vectors are used for molecular biology techniques such as site-directed mutagenesis. In general, DNA vectors that are used in many molecular biology gene cloning experiments need not result in the expression of a protein. Expression vectors are often specifically designed to contain regulatory sequences that act as enhancer and promoter regions, and lead to efficient transcription of the gene that is carried on the expression vector. Expression vectors are basic tools for biotechnology and the production of proteins such as insulin that are important for medical treatments of specific diseases like diabetes.

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